|
KMID : 0644120120250010034
|
|
Journal of Oriental Gynecology
2012 Volume.25 No. 1 p.34 ~ p.46
|
|
|
In Vitro Antibacterial Effects of Wandae-tang Aqueous Extracts and Their Combination Effects with Clindamycin against Gardnerella Vaginalis
|
|
Lee Seung-Hye
Kim Dong-Chul
|
|
|
Abstract
|
|
|
Objectives: The object of this study was to observe the in vitro antibacterial effects of Wandae-tang extracts and combination of Wandae-tang extracts and Clindamycin against Gardnerella vaginalis ATCC14018.
Methods: Antibacterial activities against Gardnerella vaginalis ATCC14018 of Wandae-tang extracts were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were also monitored at minimal inhibitory concentration(MIC) and MIC×2 levels. The combination effects of Wandae-tang extracts with Clindamycin were observed by Checkerboard microtiter assay, and the effects of bacterial growth curve was treated with Wandae-tang extracts MIC+Clindamycin MIC, 1/2 MIC and 1/4 MIC, respectively.
Results: MIC of Wandae-tang extracts and Clindamycin against Gardnerella vaginalis ATCC14018 were detected as 1.719±0.856 (0.782~3.125) mg/mL and 0.010±0.006 (0.004~0.016)㎍/mL . In addition, Clindamycin and Wandae-tang extracts were also showed marked dosage-dependent inhibition of bacterial growth, and more dramatical inhibitions were detected in Clindamycin+Wandae-tang extracts MIC treatment. Fractional inhibitory concentration index in combination of Wandae-tang extracts and Clindamycin were detected as 0.294±0.052 (0.250~0.375) at Checkerboard microtiter assay.
Conclusions: The results obtained in this study suggest that Wandae-tang extracts showed antibacterial effects against Gardnerella vaginalis ATCC14018, and they also showed dosage-dependent inhibitory effects on the bacterial growth. In addition, combination treatment of Wandae-tang extracts with Clindamycin showed more synergistically potent inhibitory effects on the growth of Gardnerella vaginalis.
|
|
|
KEYWORD
|
|
|
Gardnerella Vaginalis, Wandae-tang, Clindamycin, MIC
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|